Analytical separations and measurements: Micro/nano-chemical analysis, capillary electrophoresis, liquid chromatography, chromatographic materials and their physicochemical characterization, column technology, bioanalytical chemistry
578 Natural Sciences Complex
Buffalo NY, 14260
Phone: (716) 645-4213
Fax: (716) 645-6963
Separation science and measurements: micro/nano-chemical analysis, capillary electrophoresis, liquid chromatography, chromatographic materials and their physicochemical characterization, column technology, silica and carbon-based nanoparticles, nanomaterials in chemical analysis, bioanalytical chemistry.
The focus of our research is in the field of analytical chemistry, with particular interest on separation science; this includes the development of chromatographic media for chemical separations, detection schemes for monitoring mass-limited samples (i.e., micro scale), the use of nanotechnology in separations, and the development of new strategies to separate and analyze complex chemical or biochemical sample mixtures, such as biofluids, intracellular components, protein digests, and pharmaceutical drugs, among others.
One current research effort is centered on column technology for liquid chromatography; after all, it is within the column that the chromatographic process takes place, making the column the “heart” of the separation system. We are interested in the synthesis and physicochemical characterization of new separation media with high chemical stability, and how such characteristics influence chemical separations (i.e., selectivity). Our chromatographic materials are synthesized in different formats (i.e., particles, thin films, and monoliths), which are suitable for HPLC, supercritical fluid chromatography (SFC), and solid phase extraction. As an example, we have synthesized organo-silica nanoparticles to explore the limits of particle size and their potential use in capillary electrochromatography and HPLC. The use of submicron particle sizes also led to research in the area of ultrahigh pressure liquid chromatography (UHPLC), using pressures as high as 50,000 psi. We are also exploring the potential use of nanomaterials (e.g., carbon dots and nanodiamonds) assembled on the organo-silica supports to act as the stationary phase for chromatography.
Another area of study is the implementation of fast separation methodology with applicability to proteomics and high throughput analysis. We also use various separation technologies (e.g., LC/MS, LC/ICP/MS) to analyze environmentally relevant samples (e.g., food, fresh water, and supplements) or of biological origin (e.g., saliva, cell lysates, and tear fluid). The latter sample type allows us to explore the potential of such fluids as a mean to sample chemical species suitable for clinical analysis/diagnosis. Our efforts on developing new methodology to analyze entities of biochemical interest (proteins, DNA, drugs, metal ion complexes, etc.) have impacts in many areas (e.g., environmental, biomedical, pharmaceutical, etc.). One particular area of interest is the development of new approaches to analyze intracellular content that can facilitate investigations associated with the elucidation of biochemical pathways.