Separation science and chemical measurements: HPLC column technology and chromatographic materials, capillary electrophoresis, liquid chromatography.
The focus of our research is in the field of analytical chemistry, with particular interest on separation science. Our research group develops chromatographic media for chemical separations, detection schemes for monitoring mass-limited samples (i.e., micro scale), study the use of nanotechnology in separations, and develops new strategies to separate and analyze complex chemical or biochemical sample mixtures, such as biofluids, intracellular components, protein digests, and pharmaceutical drugs, among others. One current research effort is centered on column technology for liquid chromatography. We are interested in the synthesis and physicochemical characterization of new separation media with high chemical stability, and how such characteristics influence chemical separations (i.e., selectivity). Our chromatographic materials are synthesized in different formats (i.e., particles, thin films, and monoliths), which are suitable for HPLC, supercritical fluid chromatography (SFC), and solid phase extraction. An example is the synthesis of fluorinated phases that can impart unique selectivity in the separation and isolation of fluorine-containing compounds. Another area of study is the implementation of fast separation methodology with applicability to proteomics and high throughput analysis. We also use various separation technologies (e.g., SFC, LC/MS, LC/ICP/MS) to analyze environmentally relevant samples (e.g., food, fresh water, and supplements) or of biological origin (e.g., saliva, cell lysates, and tear fluid). The latter sample type allows us to explore the potential of such fluids as a mean to sample chemical species suitable for clinical analysis/diagnosis. Our efforts have impact in many areas; some examples include the environmental, biomedical, and the pharmaceutical fields.
- Bui, T.; Colón, L.A.; Velarde, L.; Intermolecular Interactions at the Silica-Liquid Interface Modulate the Fermi Resonance Coupling in Surface Methanol, J. Phys. Chem. Lett. 2021, 5695-5702.
- Borges-Muñoz, A.C.; Miller, D.P.; Zurek, E.; Colón, L.A., Silanization of superficially porous silica particles with p-aminophenyltrimethoxysilane. Microchem. J. 2019, 147,263-268.
- Hu, Q. and Colón, L.A., Carbon Nanomaterials in Analytical Separations, in Carbon-based Nanomaterials in Analytical Chemistry, Garcia, C.D.; Crevillén, A.G.; Escarpa, A., Eds., Royal Society of Chemistry: London, 2018: Chapter 3, pp. 69-96.
- Vujcic, S.; Santiago-Capeles, L.; Tirado-González, K.M.; Borges-Munõz, A.C.; Colón, L.A., Hafnia and Zirconia Chromatographic Materials for the Enrichment of Phosphorylated Peptides. Adv. Chromatogr. 2018, 55, 51-67.
- Borges-Muñoz, A.C.; Colón, L.A., Evaluation of an amide-based stationary phase for supercritical fluid chromatography, J. Sep. Sci. 2016, 39, 3469-3476.
- He, P.; Colón, L.A., Aga D.S., Determination of Total Arsenic and Speciation in Apple Juice by Liquid Chromatography Inductively Coupled Plasma Mass Spectrometry: An Experiment for the Analytical Chemistry Laboratory. J. Chem. Educ. 2016, 93, 1939-1944.
- Xue, Z.; Vinci, J.C.; Colón, L.A., Nanodiamond-decorated silica spheres as a chromatographic material. ACS Appl. Mater. Interfaces 2016, 8, 4149-4157.
- Vinci, J.C.; Ferrer, I.M.; Guterry, N.W.; Colón, V.M.; Destino, J.F.; Bright, F.V; Colón, L.A., Spectroscopic Characteristics of Carbon Dots (C-dots) Derived from Carbon Fibers and Conversion to Sulfur-Bridged C-dots Nanosheets. Appl. Spectrosc. 2015, 69, 1082-1090.
- Vinci, J.C.; Ferrer, I.M.; Seedhouse, S.J.; Bourdon, A.K.; Reynard, J.M.; Foster, B.A.; Bright, F.V.; Colón, L.A., Hidden Properties of Carbon Dots Revealed After HPLC Fractionation. J. Phys. Chem. Lett. 2013, 4, 239-243.
- Vinci, J.C.; Colón, L.A.; Surface chemical composition of chromatographically fractionated graphite nanofiber-derived carbon dots. Microchem. J. 2013, 110, 660-664.
Advancing Diversity in the Chemical Sciences
- Noble, E.N., et al.; Building a Networked Improvement Community: Lessons in Organizing to Promote Diversity, Equity, and Inclusion in STEM, Front. Psychol., 2021, 18. Access: https://doi.org/10.3389/fpsyg.2021.732347
- Carter, M.J.; Colón, L.A.; De Cheke Qualls, A.; Deonauth, K.; Shiakolas, P.S. Cross university collaboration for stem education and social justice” Contexts, 2021. (Access)
- Colón, L.A., Increasing Diversity in the Chemical Sciences: Experiences and Lessons, in Diversity in the Scientific Community, Volume 2: Perspectives and Exemplary Programs, Nelson, D. and Cheng, H.N., Eds; ACS Symposium Series, 1256, American Chemical Society: Washington, DC, 2017: Chapter 10, 115-124.