Thomas Szyperski


Thomas Szyperski.

Thomas Szyperski


Thomas Szyperski


UB High-Field NMR Facility

Research Interests

Nuclear magnetic resonance (NMR)-based structural biology, structural genomics and metabonomics: NMR methodology development, applications to study protein structure, dynamics and folding, metabolic profiling.

Contact Information

816 Natural Sciences Complex

Buffalo NY, 14260

Phone: (716) 645-4309

Fax: (716) 645-6963


  • Fellow im Verband der Chemischen Industrie, 1989-1991
  • Fellow of the Studienstiftung des Deutschen Volkes, 1982-1988
  • Visiting scientist, U Auckland (NZ), 1988
  • Habilitation, ETH Zürich (CH), 1998
  • Dr. sc. nat ETH, ETH Zürich (CH), 1992
  • Diploma Chemistry, TU Munich (D), 1988
  • Prediploma Chemistry, TU Munich (D), 1985
  • Prediploma Biochemistry, U Tübingen (D), 1984

Awards and Honors


Structure determination of biological macromolecules using nuclear magnetic resonance (NMR) spectroscopy, Development of Bio-NMR techniques, Structural genomics, Metabonomics.

Research Summary

Our research focuses on:

  • Development of Bio-NMR methodology.
  • Structure determinations of biological macromolecules using nuclear magnetic resonance (NMR) spectroscopy.
  • Development of NMR-based structural biology in supercooled water.
  • Investigation of cellular metabolism in support of biotechnology research.
  • Metabonomics

NMR Instrumentation:

Varian 600 and 750 MHz spectrometers (cryogenic probes)
Access to: 500 (nanoprobe), 500 (1H/ 19F/ 13C/ 15N/ 2H-probe), 400, 300 MHz spectrometers

Selected Recent Publications

  • Sathyamoorthy, B., Singarapu, K.K., Garcia, A.E., Szyperski, T. (2013) Protein Conformational Space Populated in Solution Probed with Aromatic Residual Dipolar (13)C-(1)H Couplings. ChemBioChem 14:684-688. PMID:23494854
  • Pulavarti, S.V., He, Y., Feldmann, E.A., Eletsky, A., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Kennedy, M.A., Szyperski, T. (2013) Solution NMR Structures Provide First Structural Coverage of the Large Protein Domain Family PF08369 and Complementary Structural Coverage of Dark Protochlorophyllide Oxidoreductase Complexes. J. Struct. Funct. Genomics 14: 119-126. PMID: 23963952
  • Stark, J.L., Copeland, J.C., Eletsky, A., Somerville, G.A., Szyperski, T., Powers, R. (2014) Identification of Low-molecular-weight Compounds Inhibiting Growth of Corynebacteria: Potential Lead Compounds for Anitibiotics. ChemMedChem 9:282-285. PMID:24403054
  • Sathyamoorthy, B., Parish, D.M., Montelione, G.T., Xiao, R., Szyperski, T. (2014) Spatially Selective Heteronuclear Multiple-quantum (HMQC) Spectroscopy for Bio-molecular Studies. ChemPhysChem 15:1872-1879. PMID:24789578.
  • Liu, G., Poppe, L., Aoki, K., Yamane, H., Lewis, J., Szyperski, T. (2014) High-quality NMR Structure of Human Anti-apoptotic Protein Domain Mcl-1(171-327) for Cancer Drug Design.
    PLoS One 9:e96521. PMID:24789074
  • Pulavarti, S.V., Eletsky, A., Huang, Y.J., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Szyperski, T. (2015) Polypeptide Backbone, Cβ and Methyl Group Resonance Assignments of the 24 kDa Plectin Repeat Domain 6 from Human Protein Plectin. J. Biomol. NMR Assign. 9:135-138. PMID: 24722902
  • Wu, X., Liu, R., Sathyamoorthy, B., Yamato, K., Liang, G., Shen, L., Ma, S., Sukumaran, D.K., Szyperski, T., Fang, W., He, L., Chen, X., Gong, B. (2015) Discrete Stacking of Aromatic Oligoamide Macrocycles. J. Am. Chem. Soc. 137:5879-5882. PMID:25909625
  • Eletsky, A., Pulavarti, S.V., Beaumont, V., Gollnick, P., *Szyperski, T. (2015) Solution NMR Experiment for Measurement of 15N-1H Residual Dipolar Couplings in Large Proteins and Supramolecular Complexes. J. Am. Chem. Soc. 137:11242-11245. PMID:26293598
  • Turkoglu, O., Zeb, A., Graham, S., Szyperski, T., Szender, J. B., Odunsi, K., Szyperski, T., Bahado-Singh, R. (2016) Metabolomics of Biomarker discovery in Ovarian Cancer: a Systematic Review of the Current Literature. Metabolomics 12: 60.
  • Jacobs, T. M., Williams, B., Williams, T., Xu, X., Eletsky, A., Federizon, J. F., Szyperski, T., Kuhlman, B. (2016) Design of Structurally Distinct Proteins Using Strategies Inspired by Evolution. Science 352: 687-690. PMID: 27151863